Table 1.
Fertilization rate for CO2compared with CD as type of euthanasia for IVF method A
| CO2 |
CD |
||||
| Experiment | Male strain | No. of eggs | No. (%) of 2-cell embryos | No. of eggs | No. (%) of 2-cell embryos |
| 1 | Wildtype B6 | 127 | 17 (13%) | 121 | 37 (31%) |
| 2 | Wildtype B6 | 184 | 43 (23%) | 171 | 83 (48%) |
| 3 | GE B6 | 271 | 215 (79%) | 296 | 255 (86%) |
| 4 | GE B6 | 267 | 11 (4%) | 184 | 40 (22%) |
| 5 | GE B6 | 294 | 200 (68%) | 336 | 271 (81%) |
| 6 | GE B6 | 292 | 210 (72%) | 339 | 268 (79%) |
| 7 | GE B6 | 290 | 19 (7%) | 298 | 35 (12%) |
| 8 | GE B6 | 255 | 101 (40%) | 243 | 190 (78%) |
| 9 | GE B6 | 394 | 108 (27%) | 300 | 125 (42%) |
| 10 | GE B6 | 286 | 57 (20%) | 211 | 59 (28%) |
| Total | 2660 | 981 (37%) | 2499 | 1363 (55%) | |
For all groups, the fertilization rate (no. of 2-cell embryos) was significantly (P < 0.005; Wilcoxon test) different between the CO2 and CD euthanasia methods.