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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Cancer Res. 2014 Oct 8;74(23):7024–7036. doi: 10.1158/0008-5472.CAN-14-1346

Figure 1. RT Induces GLI1 Expression In Vivo.

Figure 1

A. Schema describing tumor sections of interest that were laser capture microdissected (LCM). B. The amount of non-irradiated human GLI1 mRNA expression at the tumor margin compared to the tumor center was determined by quantitative real-time PCR (qRT-PCR) from LCM tissue. C. Irradiated and non-irradiated LCM tissue was analyzed by qRT-PCR using species specific probes for GLI1 and SHH to determine RT-induced gene fold expression over baseline. D. Two different PDX±RT. Tumors were harvested, formalin fixed, sectioned and stained with anti-EGFR or anti-Gli1 for IHC (20×, bar 100µM). Statistically significant findings were denoted: *P<0.05.