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. 2014 Nov 27;15:26. doi: 10.1186/s12867-014-0026-0

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© Fujita and Fujii; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Expression and purification of r3xFNLDD-D. (A) Coomassie Brilliant Blue (CBB) staining of the recombinant protein. The purified proteins were subjected to SDS-PAGE and CBB staining. (B) Immunoblot analysis (IB) of r3xFNLDD-D. The purified proteins were subjected to SDS-PAGE and IB with anti-Dock Ab. Supernatant: the supernatant prepared from the silkworm pupal homogenates. Precipitant: the insoluble precipitate prepared from the silkworm pupal homogenates. Elution: the eluate after affinity purification with Dock Catch Resin.