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Time-of-addition assay. 80 μM pimprinethine, 40 μM WS-30581 A or 40 μM WS-30581 B were added to RD cells at different time periods after EV71 infection. At 12 h pi, the progeny virus yield was determined (-1–0 h: viral infection period; 0–10 h: the period for virus proliferation in the cells). Values are represented as the means ± SDs. *P <0.05, compared with virus control group.
