Skip to main content
. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Virology. 2014 Aug 16;0:47–56. doi: 10.1016/j.virol.2014.07.038

Figure 3. SV40.ERΔVHR-expressing MEFs show limited growth in reduced serum conditions.

Figure 3

Cells were grown in medium containing either 10% or 1% serum for up to 12 days. Duplicate wells were counted at regular intervals and averaged. Two experiments performed with wild-type MEFs are shown separately (A). For SV40 (B) and JCV samples (C), each point on the graph represents the average number of cells from 3 different cell pools expressing the same early region construct. Error bars denote the standard error of the mean. The doubling time was calculated from time points within the exponential phase of growth and the results are summarized in (D). Each value represents the average doubling time from 3 cell pools expressing the same early region construct. MEF values are the average of 2 independent experiments. The standard error of the mean is indicated.