Fig. 3. Mechanical occlusal loading induces LH2 expression on alveolar bone side of PDL.
Eight-week-old male SD rats were subjected to 3 days of excessive occlusal loading (Kaku et al., 2005)[29]. Decalcified, paraffin embedded histology samples were prepared and the distribution of LH2 was analyzed by means of immunohistochemistry. Anti-LH2-positive cells were detected only at the bone side PDL in the experimental group (arrow heads).