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Change in thrombin:fluorescein complex fluorescence with increasing concentrations of exosite II ligand. ● CDSO3, ○ FDSO3, ▼ SDSO3, △ heparin. ΔF is the relative change in fluorescence. Line of best fit for CDSO3 (—), FDSO3 (----), SDSO3 (---) and heparin (-----). His 57 is the active site amino acid labeled by the fluorescein probe.
