Table 1.

Effects of CDSO3 on the Michaelis–Menten Parameters of Thrombin and Various Substrates^a

[CDS03] (nM)	Substrate	Km (μM)b	Vmax (mAU/min)b
0	Spectrozyme TH	2.2 ± 0.2	21 ± 0.7
30	”	1.3 ± 0.1	15 ± 0.3
105	”	1.2 ± 0.1	11 ± 0.3
300	”	0.85 ± 0.1	9.5 ± 0.2
0	S-2238	1.3 ± 0.1	10.4 ± 0.2
1	”	1.3 ± 0.2	8.8 ± 0.2
10.5	”	1.1 ± 0.2	6.2 ± 0.2
30	”	1.4 ± 0.3	5.0 ± 0.2
105	”	1.2 ± 0.3	4.6 ± 0.2
0	Spectrozyme FXa	61 ± 3	36 ± 0.6
10.5	”	42 ± 4	31 ± 0.8
30	”	40 ± 5	23 ± 0.9
105	”	40 ± 5	18 ± 0.7
203	”	39 ± 10	12 ± 1
0	Spectrozyme Pro	1.3 ± 0.2	36 ± 1
30	”	0.5 ± 0.05	23 ± 0.3
120	”	0.7 ± 0.1	21 ± 0.5
0	Spectrozyme PCa	4.5 ± 0.5	107 ± 3
30	”	7.3 ± 2.0	82 ± 4
90	”	9.1 ± 3.2	67 ± 5

^aK_M and V_MAX values of substrate hydrolysis by human thrombin were measured as described in Experimental Procedures. mAU indicates milliabsorbance units.

^bError represents ±1 SE.