Table 2.

Effects of CDSO3 on the Michaelis–Menten Parameters of Factor Xa and Various Substrates

[CDS03] (nM)	Substrate	Km (μM)b	Vmax (mAU/min)b
0	Spectrozyme FXa	64 ± 7	22 ± 0.8
10.5	”	76 ± 11	18 ± 1
30	”	41 ± 5	12 ± 0.5
105	”	52 ± 12	9.7 ± 0.8
203	”	63 ± 19	6.2 ± 0.7
0	S-2772	212 ± 21	34 ± 1
10.5	”	60 ± 5	12 ± 0.2
105	”	42 ± 15	3.4 ± 0.3
0	Spectrozyme TH	597 ± 180	80 ± 16
10.5	”	390 ± 105	50 ± 8
30	”	168 ± 75	13 ± 2
83	”	65 ± 30	6.6 ± 0.7
0	Spectrozyme Pro	188 ± 28	119 ±7
7.5	”	112 ± 8	72 ± 2
30	”	77 ± 9	53 ± 2

^aK_M and V_MAX values of substrate hydrolysis by human factor Xa were measured as described in Experimental Procedures. mAU indicates milliabsorbance units.

^bError represents ±1 SE.