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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Cancer Res. 2014 Oct 13;74(23):6913–6924. doi: 10.1158/0008-5472.CAN-14-0431

Figure 3. Different phenotypic effect of the NEFL over-expression in two neuroblastoma cells with protective and risk genotype for the SNP rs1059111.

Figure 3

(A) SH-SY5Y (TA genotype) NEFL overexpressing stable clones show longer neurites processes than pcDNA control cells whereas SK-N-AS (TT genotype) NEFL overexpressing stable clones become flatter. (B) Neurites length in SH-SY5Y cell clones were measured from the cell body to the furthest tip of the process using LAS/AF tool (Leica Mycrosistem). (C) The expression of neuronal differentiating markers is enhanced in SH-SY5Y NEFL stable clones whereas it does not change in SK-N-AS NEFL stable clones, as shown by qRT-PCR. (D) The expression of PCNA is decreased in NEFL stable clones in both cell lines. (E) NEFL overexpression impairs the growth in soft agar of SH-SY5Y stable clones as shown by a decreased number of colonies with respect to pcDNA cells. NEFL impairs the number of invading cells in 2-dimentional invasion assay in (F) SH-SY5Y and (G) SK-N-AS stable clones. The experiments have been performed in three diverse clones for each experimental point and the shown results are representative of the mean among the different clones. Optical Density (OD).