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. 2013 Oct 28;2(4):153–159. doi: 10.5812/cardiovascmed.13892

Table 1. Oligonucleotide Primer Sequences and Real-time PCR Amplification Parameters Temperature .

Gene Forward and reverse primer sequences Annealing temperature Amplicon size (bp)
ABCA1 F: 5׳-acgagattgatgaccgcctc 60 °C 109
R: 5׳-agcatccaccccactctcttc
PPAR-α F: 5׳-actcgcaggaaagactagca 60 °C 143
R: 5׳-agcagtggaagaatcggacc
GAP.DH F: 5׳-gtgccagcctcgtctcatag 60 °C 199
R: 5׳-gactgtgccgttgaacttgc