Table 1. Oligonucleotide Primer Sequences and Real-time PCR Amplification Parameters Temperature .
Gene | Forward and reverse primer sequences | Annealing temperature | Amplicon size (bp) |
---|---|---|---|
ABCA1 | F: 5׳-acgagattgatgaccgcctc | 60 °C | 109 |
R: 5׳-agcatccaccccactctcttc | |||
PPAR-α | F: 5׳-actcgcaggaaagactagca | 60 °C | 143 |
R: 5׳-agcagtggaagaatcggacc | |||
GAP.DH | F: 5׳-gtgccagcctcgtctcatag | 60 °C | 199 |
R: 5׳-gactgtgccgttgaacttgc |