Figure 5.

Comparison active site cleft of BGlu1 E386G (A) and BGlu1 E176Q/Y341A (B) in complex with cellotetraose. The sidechains of R178 (green carbons), W337 (cyan carbons) and Y341 (yellow carbons) residues noted to have significant effects on the activities of BGlu1, BGlu1 E176Q and BGlu1 E386G are shown in stick representation behind the active site surface. The cellotetraose is represented as balls and sticks. The surface representation was created in Pymol (Schrödinger LLC) and colored with the color of the underlying atoms, where carbon is grey in non-highlighted amino acid residues, oxygen is red, and nitrogen is blue.