Table II.
Kinetic Parameters of BGlu1 E176Q and its Variants for Hydrolysis of pNPGlc
| Enzyme | Km (mM) | kcat (s−1) | kcat/Km (s−1M−1) |
|---|---|---|---|
| E176Q | 0.188 ± 0.007 | 3.68 ± 0.06 | 20,000 |
| E176Q/Y341A | 0.225 ± 0.004 | 3.71 ± 0.03 | 17,000 |
| E176Q/Q187A | 0.188 ± 0.005 | 3.11 ± 0.03 | 17,000 |
| E176Q/Y341A/Q187A | 0.249 ± 0.005 | 3.86 ± 0.03 | 16,000 |
| E176Q/R178A | 0.108 ± 0.004 | 1.05 ± 0.01 | 9800 |
| E176Q/W337A | 0.144 ± 0.009 | 2.25 ± 0.04 | 17,000 |
| E176Q/Y341A/R178A | 0.135 ± 0.006 | 1.28 ± 0.02 | 9500 |
| E176Q/Y341A/W337A | 0.204 ± 0.012 | 3.03 ± 0.06 | 15,000 |
| E176Q/Y341A/R178A/W337A | 0.333 ± 0.006 | 1.98 ± 0.01 | 6000 |