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The effects of treatment with DTT and a chaotropic agent. SRCD spectra (in black) of (a) polyproline and (b) collagen in aqueous solution (solid), incubated with 5 mM DTT (dotted), and with 4M urea (dashed). The corresponding HT curves (in gray) show the wavelength cutoff values for the urea-containing samples are at around 198nm, but that the negative peaks at around 200 nm can be accurately measured.
