Thermodynamic parameters. Thermodynamic parameters at 25°C. ΔG‡ (green), ΔH‡ (blue), and TΔS‡ (yellow) for the temperature-sensitive (TS) reaction, ATP hydrolysis, and Pi release were calculated from Figure 4(a,b), while that for ATP binding at 200 µM ATP was calculated from Watanabe et al.25