Table I.
Comparison of the Tm Values for the Unfolding of moPrP 121–231 Fragment in the Presence of Different Nucleic Acids at a Constant [Nucleotide]/[PrP] Ratio of 4 at pH 5
| Nucleic acids | No. of nucleotides (nt) | Tm (°C) at a fixed [nucleotide]/[PrP] = 4 ratios |
|---|---|---|
| MoPrP(121–231) in Buffer, pH5 | — | 65.4 ± 0.2 |
| MoPrP(121–231) in Buffer, pH6 | — | 65.8 ± 0.3 |
| MoPrP(121–231) in Buffer, pH7.2 | — | 66.5 ± 0.4 |
| Polyadenylic acid [PolyA] | 910 | 49.0 ± 0.5 |
| Polycytidylic acid [PolyC] | 2500 | 53.0 ± 0.6 |
| Polyuradylic acid [PolyU] | 2727 | 48.0 ± 0.4 |
| ds poly (dA-dC).(dG-dT) [mDNA] | 5316 | 45.0 ± 0.3 |
| Transfer RNA [tRNA] | 80 | 45.0 ± 0.5 |