Fig. 2. Developmental expression of AeaETH-receptors.
ETHR transcripts in CC-CA complexes during the pupal stage were measured using q-PCR. Levels of ETHR mRNA are expressed as copy number of AeaETHR mRNA/10,000 copies of rpL32 mRNA. Each q-PCR data point is the mean ± S.E.M. of two independent biological replicates of 20 CA-CC complexes. Significant differences (p< 0.05) were determined by one-way ANOVA followed by Tukey’s test.