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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Insect Biochem Mol Biol. 2014 Sep 23;54:98–105. doi: 10.1016/j.ibmb.2014.09.006

Fig. 4. Tissue localization of AeaETH-receptor and effect of ETH on different CA preparations.

Fig. 4

Corpora allata (CA) and corpora cardiaca CC were individually dissected from pharate adult female mosquitoes. A) Expression of the AeaETHRs and four additional marker genes in CC and CA. AKH (1): adipokinetic hormone; CCAP (2): crustacean cardioacceleratory peptide; JHAMT (3): juvenile hormone acid methyltransferase; EPOX (4): epoxidase; ETH receptors (5): ecdysis triggering hormone receptors. Transcripts levels are expressed as percentage of the maximum value found in the two glands. Each q-PCR data point is the mean ± S.E.M. of two independent biological replicates of 20 tissue samples. B) Different types of CA preparations were incubated with AeaETH-1 (10−9 M), and JH biosynthesis was evaluated by HPLC-FD. Each data point represents the means ± S.E.M. of 2–4 independent determinations. Asterisks denote significant differences (unpaired t-test; *P ≤ 0.05;**P ≤ 0.01; *** P ≤ 0.001).