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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Cancer Res. 2014 Oct 8;74(23):6771–6783. doi: 10.1158/0008-5472.CAN-14-0037-T

Fig. 4. CD47 blockade enhances antigen-dependent T cell killing of 15-12RM fibrosarcoma when combined with irradiation.

Fig. 4

(A) 15-12RM target cells were seeded into 16-well plates. RT-1-derived effector T cells were co-cultured with target cells at a ratio of 1:5, and target cell growth and viability was dynamically monitored using the RT-CES system. Target cell viability monitored by surface impedance is presented as a normalized cell index (N=3 in triplicate, *p<0.05). Cells were plated into 96-well plates and effector and or targets cells were treated with 10 µM CD47 Morpholino (CD47 M; A, C and D). Mismatched morpholino (MM A and B) or mouse antibody to CD47 clone 301 (D), and LDH release was quantified after 24 h as a measure of cell cytotoxicity, N=3 *p<0.05.