Figure 5. Functional annotation of the three asthma associated regions by FAIRE-PCR analysis.
Formaldehyde assisted isolation of regulatory enrichment (FAIRE) was performed at three association regions nearby FADS2, NAGA and F13A genes in Beas-2B, A549 and Jurkat cell lines by real-time PCR analysis. The graph depicts the relative FAIRE signal at each locus normalized to input and negative control regions. Each locus contains three SNPs nearby eQTL signals. Mean±SD are from two to four repeats for each SNP. **p<0.01 (unpaired one way t test.)