Skip to main content
. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Exp Cell Res. 2014 Sep 4;328(2):388–400. doi: 10.1016/j.yexcr.2014.08.037

Fig. 6. Fetuin-A mediated trafficking of vinculin and histone H2A to the membrane fraction of adhered and spread cells.

Fig. 6

Fetuin-A transfected BT-549 cells and empty vector transfection controls were dislodged from culture flasks with EDTA and allowed to adhere to 145 mm petri dishes pre-coated with either laminin or fibronectin (40 μg/ml) and incubated for 30 min at 37°C in a humidified CO2 incubator. The cells were lysed and fractionated into nuclear chromatin bound, cytosolic and membrane associated proteins as described in Materials and Methods. In panel A, the cytosolic and membrane fractions from cells incubated on laminin coated dishes were resolved in NuPAGE gels, transferred to nitrocellulose and probed for vinculin, Akt1, FLAG-fetuin-A, and EGFR. In panel B, the membrane fractions from cells incubated on fibronectin coated dishes were probed for vinculin, FLAG-fetuin-A, histone H2A and EGFR.