Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Nov 21;14:857. doi: 10.1186/1471-2407-14-857

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Jiang et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

ERK1/2 activation is B- and CRAF-dependent in GHM cells. (A) Fold change of ARAB, BRAF and CRAF mRNA after silencing with specific siRNAs in HoMel-A1 and HoMel-L1 cells as compared to scrambled control siRNA (siNeg). Fold changes are expressed as a range, with the standard deviation of the ΔΔCT value incorporated into the fold change calculation. (B, D) Contribution of individual RAF kinases to ERK1/2 activation in HoMel-A1 and HoMel-L1 cells after silencing with specific siRNAs or scrambled control siRNA as assessed by Western blot analysis. Representative images of one of three independent experiments are shown. (C, E) Quantification of total ERK1/2-normalized ARAF, BRAF, CRAF and P-ERK1/2 protein levels in individual silencings. Shown are the mean ± s.e. of three independent experiments.