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. Author manuscript; available in PMC: 2015 Nov 28.
Published in final edited form as: J Control Release. 2014 Aug 23;194:71–81. doi: 10.1016/j.jconrel.2014.08.013

Figure 8.

Figure 8

Cytotoxicity of free DOX and DOX-loaded PTSL and TSL at (A) 100 nM and (B) 500 nM DOX. Free DOX or DOX-loaded TSL and PTSL were added to cells for 4 hr. To study effects of heating, equivalent solutions were heated to 43°C for 5 min prior to exposure to cells. Following exposure, solutions were replaced with pH 7.5 media free of liposomes/drug. Cells were incubated for 192 hr after which cell viabilities were determined via MTT assay. Data shown are averages ± SD for n=6 replicates. a p=0.02 between heated/unheated TSL, 100 nM; b p=0.003 between heated/unheated PTSL, 100 nM; c p=0.04 between heated PTSL/heated TSL, 100 nM; d p=0.001 between unheated PTSL/unheated TSL, 500 nM; e p=0.00002 between heated/unheated PTSL, 500 nM; n.s. indicates no significant difference observed between heated/unheated TSL, 500 nM.