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. Author manuscript; available in PMC: 2015 Nov 28.
Published in final edited form as: J Control Release. 2014 Aug 23;194:71–81. doi: 10.1016/j.jconrel.2014.08.013

Figure 9.

Figure 9

Cytotoxicity of DOX-loaded and empty PTSL at varying pH. DOX-loaded or empty PTSL in growth media at pH 7.5, 7, and 6.5 were added to cells for 4 hr after which solutions were replaced with pH 7.5 media free of liposomes/drug. Cells were incubated for 192 hr after which cell viabilities were determined via MTT assay. Data shown are averages ± SD for n=6 replicates. a p=0.01 between pH 7 and pH 6.5, 175 nM DOX-PTSL; b p=0.001 between pH 7.5 and pH 6.5, 175 nM DOX-PTSL; c p=0.02 between empty PTSL and DOX-PTSL, 175 nM, pH 6.5; d p=0.0002 between pH 7.5 and pH 7, 250 nM DOX-PTSL; e p=0.003 between empty PTSL and DOX-PTSL, 250 nM, pH 7; f p=0.0002 between pH 7.5 and pH 6.5, 250 nM DOX-PTSL; g p=0.016 between empty PTSL and DOX-PTSL, 250 nM, pH 6.5. No significant differences were observed as a function of pH in groups administered the same concentration of empty PTSL.