Figure 2.

Seeding on Netrin-1-immobilized scaffolds led to a higher percentage of polarized RGCs.
RGCs seeded on control PLA scaffolds (A) and Netrin-1 immobilized scaffolds (B) were stained for β3 tubulin and analyzed for axon polarization and were classified as polarized or bipolar (C). A significant increase in the percent of polarized RGCs was observed on cells seeded on Netrin-1-immobilized scaffolds (mean ± standard error of mean; *p<0.05 by unpaired Student t-test). Scale bar A, B: 200 μm A′,B′: 75 μm.