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Western blotting of total protein extracts of C. neoformans ISC1-PMA1::HA (WT) and Δiscl-PMA1::HA (Δisc1) using anti-HA monoclonal antibody. The Δisc1 strain showed increased production of Pma1-HA at both acidic and neutral pH after 4 and 16 hours of growth. Giantin and β–actin were used as loading control.
