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. Author manuscript; available in PMC: 2015 Nov 3.
Published in final edited form as: FEBS Lett. 2014 Sep 18;588(21):3932–3938. doi: 10.1016/j.febslet.2014.09.005

Figure 4.

Figure 4

Addition of C6-phytoceramide increased the synthesis and oligomerization of Pma1 in the Δisc1 strain in a concentration-dependent manner. (A) SDS-PAGE of total protein extracts of the C. neoformans ISC1-PMA1::HA (WT) and Δisc1-PMA1::HA after the addition of 5 to 20 µM of phytoceramide using anti-HA monoclonal antibody. (B) Native-PAGE of total protein extracts of the ISC1-PMA1::HA (WT) and Δisc1-PMA1::HA after the addition of 5 to 20 µM of phytoceramide using anti-HA monoclonal antibody. Both gels were performed 2 hours after the addition of phytoceramide and in acidic environment (pH=4), values show the concentration of phytoceramide in the medium.