Table 1.
Chemokine Expression Using Nanostring Technology
| Female | Day 0 WT | Day 0 KO | Day 0 KO:WT | Day 2 WT | Day 2 KO | Day 2 KO:WT | Day 4 WT | Day 4 KO | Day 4 KO:WT |
|---|---|---|---|---|---|---|---|---|---|
| Cxcl1 | 18 | 9 | 0.48245614 | 42 | 135 | 3.229181665 | 69 | 1054 | 15.3712828 |
| Cxcl10 | 183 | 107 | 0.583755792 | 91 | 72 | 0.786341678 | 36 | 488 | 13.50886427 |
| Cxcl2 | 33 | 31 | 0.926337943 | 172 | 677 | 3.948107982 | 474 | 9683 | 20.42041884 |
| Cxcl3 | 23 | 7 | 0.292942743 | 211 | 1320 | 6.250437911 | 212 | 4115 | 19.42882772 |
| Cxcl5 | 12 | 10 | 0.808474576 | 49 | 41 | 0.81970862 | 114 | 154 | 1.346978046 |
| Cxcl9 | 24 | 7 | 0.311016949 | 11 | 14 | 1.289868668 | 7 | 21 | 2.962603878 |
| Cxcr4 | 5648 | 1518 | 0.268678675 | 1482 | 1483 | 1.001025959 | 2930 | 12318 | 4.203348246 |
*
Number of mRNA chemokine transcripts from female WT and Dusp1−/− cell lysates harvested at day 0 (primed with M-CSF and RANKL), Day 2 and Day 4 (primed and stimulated with 50ng/ml LPS). Bolded headings are ratio of KO (Dusp1−/−) transcripts to WT at each time point. Rows highlighted in red indicate chemokines which signal through the same receptor. Values are presented as absolute number of transcripts and ratio normalized to internal reference controls.