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. Author manuscript; available in PMC: 2015 Nov 11.
Published in final edited form as: J Mol Biol. 2014 Sep 2;426(22):3811–3826. doi: 10.1016/j.jmb.2014.08.023

Figure 1. Sar1 transforms GUVs into tubular structures of heterogeneous morphologies.

Figure 1

(A) Naked GUVs embedded in vitreous ice shows a smooth morphology compared to (B) the textured appearance of GUVs coated with Sar1 (arrows). Scale bar = 500 nm. (C) Human wild type Sar1 incubations with GUVs in presence of GTP, GMPPNP, and GMPPCP. Samples were adhered to glow-discharged EM grids and stained with 2% uranyl acetate (UAc) for imaging by EM. Scale bar = 200 nm. (D) Similar incubations were performed using GTPase-defective Sar1H79G mutant isoform. Scale bar = 200 nm. (E) CryoEM of tubular structures generated by Sar1H79G incubations with GUV and GTP. Scale bar = 100 nm. (F) Diameter distribution of Sar1H79G tubes determined from the cryoEM data.