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. 2014 Oct 24;2(10):e12175. doi: 10.14814/phy2.12175

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© 2014 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Dynamics of intracellular cAMP levels during EC barrier restoration. (A) Intracellular cAMP levels during the recovery. EC monolayers were treated with thrombin (Thr; 0.3 IU/mL) or vehicle (C; control) for indicated time periods and cAMP levels were measured by ELISA (Mean ± SEM of three experiments of independent cell preparations, *P < 0.05). (B) EC monolayers were treated with cytochalasin D (cyto; 1 μmol/L), forskolin (FSK; 10 μmol/L) or vehicle (C; control) for indicated time points and cAMP levels were measured by ELISA. (Mean ± SEM of three experiments of independent cell preparations, *P < 0.05). (C) Effect of AC inhibition on EC barrier recovery. EC monolayers were exposed to thrombin (Thr; 0.3 IU/mL) in the absence or presence of AC inhibitor MDL12330 (10 and 25 μmol/L) or vehicle (control) as indicated and albumin flux (permeability) was measured. Mean ± SEM of three experiments of independent cell preparations; *P <0.05 versus control, ^#P <0.05 versus Thr alone.