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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Insect Biochem Mol Biol. 2014 Sep 19;54:112–121. doi: 10.1016/j.ibmb.2014.09.004

Table 1.

Cry11Aa has a lower affinity to midgut membrane prepared from the G30 larvae than those from wild type.

WT midgut G30 midgut
Kd (nM)a 13.4 30.5

Binding affinity of WT and G30 was tested with early fourth-instar larvae midguts. Specific binding of biotin-labeled Cry11Aa to BBMV was obtained from total binding minus nonspecific binding. Cry11Aa binding affinity for G30 BBMV was slightly reduced. Kd values were obtained from the concentration corresponding to half the saturation response of specific binding.

a

The Kd values reported are the mean of three separate experiments.