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. Author manuscript; available in PMC: 2015 Oct 5.
Published in final edited form as: Mol Biochem Parasitol. 2014 Oct 5;197(0):36–42. doi: 10.1016/j.molbiopara.2014.09.009

Fig. 1.

Fig. 1

Expression and characterization of T. gondii canonical H2B. (A) Recombinant H2Ba (rH2Ba) and rH2Bv (renamed H2B.Z) were resolved in SDS-PAGE, blotted to nitrocellulose membrane (stained with Ponceau red), and analyzed by Western blot against mouse rH2Ba (αrH2Ba). (B) Competition ELISA was performed with αrH2Ba serum incubated with increasing quantities of rH2Ba against recombinant proteins. The panel corresponds to the affinity of the antibody, indicated as the title, respect to the specific recombinant protein immobilized (1 µg/ml of rH2Ba). The anti-rH2Ba serum sample was pre-incubated with different quantities of rH2Ba, rH2B.Z and rROP2 recombinant proteins. (C) Whole tachyzoite lysate (Tz) was analyzed by Western blot using αrH2Ba and αrH2B.Z antibodies. The αrH2Ba recognized a single band at expected size, with a lower MW than H2B.Z.