Fig. 4.
Genome localization. Chromatin immunoprecipitation (ChIP) was carried out using αrH2A.X, αrH2A.Z, αrH2B.Z or αrH2A.X or αrH2Ba antibodies, and preimmune serum (Pre). Immunoprecipitated DNA was then analyzed by qPCR with primers of SAG1, SAG2A, β-tubulin (tub), DHFR, ElF4, BAG1 and LDH2 promoters as well as TgIRE100 and TASL_IV, both from different regions on TAS element. In the figure ChIP enrichment relative to the input DNA (taken as 1) is shown.