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. Author manuscript; available in PMC: 2015 Nov 6.
Published in final edited form as: Cell Stem Cell. 2014 Oct 9;15(5):605–618. doi: 10.1016/j.stem.2014.09.008

Figure 2. Hepatocyte-derived liver progenitors cells are isolated with MIC1-1C3 antibody.

Figure 2

A) Dissociated livers were FACS sorted with gates applied for FSC/SSC (to include ductal cells, as shown), pulse width (not shown), PI− (not shown), and MIC1-1C3+ CD11b− CD31− CD45−. MIC1-1C3+ cell were separated based on mTomato fluorescence (mature hepatocyte origin). Without injury mTomato+ cells were a trace component of MIC1-1C3+ population but increased with injury. B) The percentage of ductal cells derived from mTomato-marked hepatocytes is plotted against the number of days of DDC injury. Hepatocyte-derived MIC1-1C3+ ductal progenitors emerged after approximately 4 weeks injury. C) FACS isolated populations were fixed and nucleus to cytoplasmic ratios and D) cell diameter and were examined for each population (pairwise t-test, p<0.001 ***, p<0.0001****). E) Representative H&E staining (bars = 10μm) and F) transmission electron microscopy from directly isolated cells from each population (bar size indicated). The arrow indicates a membrane bound structure in a lysosome adjacent to mitochondria.