Skip to main content
. Author manuscript; available in PMC: 2015 Dec 10.
Published in final edited form as: Gene. 2014 Oct 5;553(1):49–56. doi: 10.1016/j.gene.2014.10.001

Fig. 5. Co-transfection assays using both pPacSp1 or pPacSp3 or pPacSp4 vectors and SLC52A1 minimal promoter luciferase construct in Drosophila SL-2 cells.

Fig. 5

SL-2 cells were co-transfected with 3μg of the minimal promoter construct in pGL3-basic and 2μg of either control plsmid pPac0 or Drosophila Sp expression plasmids (pPacSp1 or pPacSp3 or pPacSp4). At 48 h post-transfection, cells were harvested and firefly luciferase activity was assayed as described in MATERIALS AND METHODS. Firefly luciferase activity was normalized relative to Renilla luciferase activity and results were expressed as fold over pGL3-basic expression; data are presented as mean ± SE of at least 3 independent experiments.