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. Author manuscript; available in PMC: 2015 Dec 10.
Published in final edited form as: Gene. 2014 Oct 5;553(1):49–56. doi: 10.1016/j.gene.2014.10.001

TABLE-1. Primer sequences used in PCR for deletion of 5′-regulatory region of SLC52A1 promoter.

Sequence (5′-3′) Position
CGGGGTACCGCTCTAGCCTGGGTGAC −310 to −292
CGGGGTACCAGCTGTGTGGGCTCTGG −234 to −216
CGGGGTACCGGATCCGGAGGTGCGG −146 to −131
CGGGGTACCAACTCGACCTGCCTCAGC −23 to −7
CGGGGTACCTGAGTGTCGCAAAAGCGC +2 to +20
CCGCTCGAGGCGACAGAACGAGATTCG +134 to 150

KpnI and XhoI restriction sites are shown by bold and underline, respectively. The relative position of primers is based on numbering the start site of transcription of SLC52A1 as position+1.