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. Author manuscript; available in PMC: 2015 Nov 15.
Published in final edited form as: J Neuroimmunol. 2014 Aug 27;276(0):180–186. doi: 10.1016/j.jneuroim.2014.08.618

Fig. 2. IL-7 stimulation leads to a higher increase of IFN-γ production in NK cells of MS patients.

Fig. 2

(A) Gating strategy for NK subpopulations. NK cells in PBMCs are analyzed by flow cytometry and gated based on surface markers CD3 and CD56, which express CD3−CD56dim or CD3−CD56bright. (B) Representative IFN-γ expressing NK cells from one MS patient and one HC with or without IL-7 stimulation are shown. PBMCs from MS patients and HCs were cultured with or without IL-7 for 72 hour. Intracellular staining of IFN-γ gating on CD3−CD56dim and CD3−CD56bright NK cells were analyzed by flow cytometry. (C) Increase in the percentage of IFN-γ-expressing CD56dim and CD56bright cells was calculated as the difference between unstimulated and IL-7 stimulation. (D) Representative intracellular IFN-γ intensity on NK cells from one MS patient and one HC is shown. (E) The changes in IFN-γ MFI on CD56dim and CD56bri cells were calculated as the difference between unstimulated and IL-7 stimulation. Data from 10 HCs and 10 MS are summarized. 5 relapsing remitting patients, 3 secondary progressive and 2 primary progressive patients were included in this study. HC were relatives of MS patients. *p < 0.05; **p < 0.01. ns, not significant. MFI, mean fluorescence intensity. CD56bri indicates CD56bright.