Table 1.
Summary data of microsatellites La Gomera Giant lizard ( Gallotia bravoana ) for all loci (A) for wild individuals (B) and for captive samples (C)
| A) Loci | Locus | Repeat motif | Tª (ºC) | Primers sequence 5′-3′ | Allele range | N | N A | N AR | H O | H E | F IS 1 | GenBank No. | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GBR5 | (CA)10 | 60 | F: ATATTCATCCTCCCCGCACA | 177 | 90 | 1 | - | - | - | - | JX661253 | ||
| F: GCATTGCGGTGAAAAAGG | |||||||||||||
| GBR9 | (GT)17 | 60 | F: TGGAGGCTTCTCTTGAGGCAAGA | 138-160 | 98 | 4 | 3.9 | 0.143 | 0.153 | 0.066* | JX661254 | ||
| F: CCCCCTGCCTTATGAGTTTCG | |||||||||||||
| GBR11 | (GTCT)6 (ATCT)12 | 60 | F: CTTAACCGTCTGGTTTGCATTA | 196-215 | 96 | 2 | 2.0 | 0.000 | 0.021 | 1.000* | JX661255 | ||
| F: ACTGCACCCCATAGTTGTCTTT | |||||||||||||
| GBR15 | (CA)27 | 60 | F: ACTGGGGCTCAGTCTTTGTTT | 140 | 91 | 1 | - | - | - | - | JX661256 | ||
| F: GCGTGTCTTGTGTATATGGAATC | |||||||||||||
| GBR16 | (CA)14 | 60 | F: GCAGATTTAATGGAACCTGGAG | 224-238 | 91 | 2 | 2.0 | 0.011 | 0.033 | 0.664* | JX661257 | ||
| F: CAACAAAATGTGGAGTTTTAGCC | |||||||||||||
| GBR20 | (GT)16 (GTAA)7 | 60 | F: CCACAACAAAACAAATGCAA | 190-217 | 97 | 5 | 4.9 | 0.051 | 0.081 | 0.362* | JX661258 | ||
| F: GTCAGATCGACCCTCTCAGC | |||||||||||||
| GBR24 | (CA)28 | 60 | F: ACTTGCAGACTATTTTGGGTT | 129-167 | 96 | 4 | 3.9 | 0.531 | 0.512 | -0.039* | JX661259 | ||
| F: ACTCGCATCCTTCTGTTACAA | |||||||||||||
| GBR26 | (CT)15 (CA)13 | 60 | F: TGGCCACACGAGATTATTCA | 103-164 | 90 | 7 | 7.0 | 0.100 | 0.139 | 0.282* | JX661260 | ||
| F: ATATCGGGCCHTTTCACA | |||||||||||||
| GBR28 | CA)26 | 60 | F: ACAACACGCCTCAGTTCACA | 195 | 89 | 1 | - | - | - | - | JX661261 | ||
| F: GCTGCCTTGAGTGAGTCTCC | |||||||||||||
| GBR29 | (GT)110 | 60 | F: GGCGTGCTTGTGTATAGGAA | 132-174 | 97 | 3 | 2.9 | 0.010 | 0.031 | 0.666* | JX661262 | ||
| F: CCCAGCAGGGTTGCTTAG | |||||||||||||
| GBR30 | (CA)13 | 60 | F: CGCACACTTATCCTGTCGTG | 198-206 | 98 | 2 | 2.0 | 0.010 | 0.030 | 0.664* | JX661263 | ||
| F: GACAGTGAGTCATGTGTGCATTT | |||||||||||||
| Mean (all indiv.)2 | 84.9 | 3.6 | 3.6 | 0.107 | 0.125 | 0.142* | |||||||
| B) Wild individuals | GBR9 | 56 | 3 | 2.9 | 0.180 | 0.179 | -0.90 | ||||||
| GBR20 | 56 | 2 | 2 | 0.053 | 0.052 | -0.019 | |||||||
| GBR24 | 54 | 2 | 2 | 0.494 | 0.490 | -0.127 | |||||||
| GBR26 | 53 | 4 | 4 | 0.074 | 0.073 | -0.017 | |||||||
| GBR29 | 59 | 2 | 1.9 | 0.036 | 0.036 | 1.000* | |||||||
| GBR30 | 56 | 2 | 1.9 | 0.018 | 0.018 | 0.000 | |||||||
| Mean (all wild indv.) | 55,7 | 2.5 | 3.1 | 0.143 | 0.141 | -0.052 | |||||||
| C) Captive samples | GBR9 | 42.0 | 3 | 2.98 | 0.114 | 0.115 | 0.382 | ||||||
| GBR11 | 41.0 | 2 | 1.99 | 0.048 | 0.048 | 1.000 | |||||||
| GBR16 | 36.0 | 2 | 2.00 | 0.080 | 0.081 | 0.660 | |||||||
| GBR20 | 41.0 | 5 | 4.62 | 0.117 | 0.119 | 0.592 | |||||||
| GBR24 | 42.0 | 4 | 3.71 | 0.475 | 0.481 | -0.040 | |||||||
| GBR26 | 37.0 | 7 | 6.92 | 0.226 | 0.229 | 0.413 | |||||||
| GBR29 | 42.0 | 2 | 1.86 | 0.023 | 0.024 | 0.000 | |||||||
| GBR30 | 42.0 | 2 | 1.98 | 0.046 | 0.047 | 1.000* | |||||||
| Mean (all captive indv.) | 40.4 | 3.4 | 3.26 | 0.141 | 0.143 | 0.511 |
N= number of individuals assayed. N A= number of alleles per locus. N AR=allelic richness standardized to the smallest sample size using the rarefaction method of FSTAT 2.9.3 [73]. expected (H E) and observed (H O) heterozygosities. F IS = Wright’s statistics.
1Bold F IS values are significant probability estimates after q-value correction (*p<0.05).
2The mean values were calculated only with the polymorphic loci data.