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. Author manuscript; available in PMC: 2014 Dec 3.
Published in final edited form as: Cell Signal. 2014 Sep 19;26(12):3027–3035. doi: 10.1016/j.cellsig.2014.09.010

Figure 4. Ang-(1-7) attenuates MGA-induced cell hypertrophy in the NRK-52E cells.

Figure 4

Left: MGA treatment for 48 hours significantly increased 3H-leucine incorporation approximately 180% of control. Ang-(1-7) (A7, 100 nM) significantly reduced the extent of hypertrophy induced by MGA. The Ang-(1-7) receptor antagonist D-Ala7-Ang-(1-7) (DAL, 10 μM), reversed the inhibitory effects of Ang-(1-7) on MGA-induced hypertrophy. The ERK 1/2 inhibitor PD98059 (PD, 1 μM), abolished the cellular hypertrophy induced by MGA. Ang-(1-7) and PD98059 exhibit no additive effects on hypertrophy. The DAL antagonist alone or the AT1 antagonist losartan (LOS) alone did not influence the MGA-dependent hypertrophy. 3H-leucine incorporation is expressed as percentage (%) from control cells for each experiment. Data are the mean ± SEM from 5 different cell passages. Inset: MGA treatment for 48 hours increased cell area as measured in immunofluorescent images. Ang-(1-7) (A7, 100 nM) and PD98059 (PD, 1 μM), reversed the increase in cell area induced by MGA.*P < 0.05 vs. control, #P < 0.05 vs. MGA-treated cells.