Western blot analysis of ERK1/2 with MGA treatment for 48 hours. MGA increased the phosphorylation of ERK1/2. Ang-(1-7) attenuated that MGA-induced phosphorylation which was blocked by D-Ala7-Ang-(1-7) (DAL, 10 μM). Inhibitors to TGF-β1 receptor kinase (SB, 1 μM) and ERK1/2 (PD, 1 μM) abolished ERK1/2 phosphorylation. The AT1 receptor antagonist losartan (LOS, 1 μM) did not inhibit the MGA-induced ERK1/2 activation. Data are the mean ± SEM from 3 different cell passages. *P < 0.05 vs. control, #P < 0.05 vs. MGA.