Figure 1.
VEGF-C treatment down-regulates VEGFR-3 protein and mRNA expression in SV-LECs and hLECs. Western blot analysis of VEGFR-3 in: (A) SV-LECs and hLECs left untreated or stimulated with VEGF-C for 24 hours; (B) vECs at the indicated times after VEGF-C (50 ng/mL) stimulation; and (C) serum-starved SV-LECs exposed to increasing concentrations of VEGF-C for 24 hours. (D) Quantitative real-time PCR analysis of VEGFR-2 and -3 as well as GAPDH mRNA levels in SV-LECs stimulated with VEGF-C for 24 hours.
