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. Author manuscript; available in PMC: 2015 Nov 20.
Published in final edited form as: Chem Biol. 2014 Oct 9;21(11):1445–1451. doi: 10.1016/j.chembiol.2014.08.019

Figure 1. Characterization and Structure of the Homotetravalent Allergens.

Figure 1

(A) Structures of the haptens DNP and dansyl used in the tetravalent allergens. (B) The affinity of each hapten was determined for its specific IgE using a fluorescence quenching technique (Supplemental Information). The Kd of DNP for IgEDNP was 22 ± 2 nM and the Kd of dansyl for IgEdansyl was 54 ± 4 nM. Data represents the means ± SD of triplicate experiments. (C) Structures of the synthetic allergens HmTA [DNP4], HmTA [dansyl4], and HmBA [DNP2].