Skip to main content
. Author manuscript; available in PMC: 2015 Nov 20.
Published in final edited form as: Chem Biol. 2014 Oct 9;21(11):1445–1451. doi: 10.1016/j.chembiol.2014.08.019

Figure 3. Two Allergen Model Reveals Complex Relationship Between IgE Cross-linking and Degranulation.

Figure 3

RBL cells were primed with mixtures of IgEs (50/50/0, 25/25/50, 15/15/70, 10/10/80, 5/5/90, and 1/1/98 IgEDNP/IgEdansyl/IgEcyclin A) at a saturating total IgE concentration of 1 μg/mL. The cells were washed and then exposed to mixtures of HmTA [DNP4] with HmTA [dansyl4] ranging from 0.5 to 1000 nM concentration of each allergen. Selected data shown here with complete results as Figure S2. Data represents the means ± SD of triplicate experiments.