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. Author manuscript; available in PMC: 2015 Apr 30.
Published in final edited form as: Science. 2014 Oct 31;346(6209):1256898. doi: 10.1126/science.1256898

Fig. 5. Mitochondrial TMF and golgin-84 relocate Golgi membrane proteins.

Fig. 5

(A) Confocal micrographs of COS cells expressing TMF-mito (HA) and stained for a Golgi enzyme, GalNAc-T2 and a Golgi marker, GCC88 (trans), without, or following, six hours nocodazole treatment as indicated. (B,C) COS cells expressing the indicated golgin-mito were treated with nocodazole for 6 hours and costained for GalNAc-T2 and a Golgi marker, GCC88 (trans). (D) COS cells expressing golgin-84-mito were treated with 0.5 μM nocodazole for 6 hours and labelled for two of the endogenous Golgi proteins GalNAcT2, TGN46 or COPI as indicated. In all cases, closed arrows indicate Golgi ministacks positive for both Golgi markers, with these being distinct from mitochondria, whilst open arrows indicate structures on mitochondria positive for GalNAcT2 but not the other marker. (E) COS cells coexpressing golgin-84ΔCterm-FRB and FKBP-MAO were treated with 0.5 μM nocodazole for three hours followed by 200 nm rapamycin (and nocadazole) direct dimerization, and then fixed as indicated and stained for endogenous GalNAcT2 and TGN46. Closed arrows indicate Golgi ministacks positive for both markers, and open arrows GalNAcT2 captured onto mitochondria after 15 minutes. Scale bars 10 μm.