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. 2014 Aug 12;11:146. doi: 10.1186/1743-422X-11-146

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© Shaukat et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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VIDISCA PCR fragments visualized on a 3% metaphor gel which were generated after amplification in a single round PCR of 40 cycles. Lane 1: control supernatant from uninfected RD cells; Lane 2–6: PCR product of cultured viruses harvested from L20B cells; Lane 7–11: PCR product of cultured viruses harvested from RD cells; Lane 12: 25 base pair molecular weight marker.