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. Author manuscript; available in PMC: 2015 Nov 10.
Published in final edited form as: Dev Cell. 2014 Oct 23;31(3):319–331. doi: 10.1016/j.devcel.2014.08.024

Figure 3. Hemicentin mutants have delayed ventral epidermal BM breaching.

Figure 3

(A) Time-lapse analysis of wild-type worm shows breach of both the gonadal and ventral BMs (laminin::GFP, white; arrowheads, top). In contrast, a him-4(rh319) worm at the P6.p two-cell stage (bottom) initially breaches only the gonadal BM (arrowheads, note the GFP signal present from ventral BM). The diagram on the right schematically depicts this data from both the lateral and ventral perspective. (B) Time-lapse analysis of a wild-type worm (top) and him-4(rh319) worm (bottom) reveals that the him-4 mutant breaches the ventral BM in a delayed manner at the P6.p four-cell stage (images correspond with Movie S3). (C) Ventral BM breach assessed in relation to gonadal breach. Representative images of each category are pictured. (*p<0.05, **p<0.005, ***p<0.0005, ≥17 animals for each category, Fisher’s exact test, error bars denote 95% confidence intervals with a continuity correction). (D) The graph depicts BM removal in representative animals. (E) The average rate of BM opening (*p<0.05, Students t-test, ≥7 timelapses for each category, error bars represent SEM). Scale bars, 5μm. See also Figure S3.