Table 1. Four oligonucleotides for the construction of the Aβ42 gene.
No. | Sequence | Length |
AD-F1 | 5'-GGGGATCCGATGCAGAATTCCGACATGACTCAGGATATGAAGTTCATCA-3 | 49 |
AD-F2 | 5'-GATATGAAGTTCATCATCAAAAATTGGTGTTCTTTGCAGAAGATGTGGGT-3' | 50 |
AD-R1 | 5'-GGCTCGAGCTACGCTATGACAACACCGCCCACCATGAGTCCAATGATTG-3 | 49 |
AD-R2 | 5'-ATGAGTCCAATGATTGCACCTTTGTTTGAACCCACATCTTCTGCAAAGAA-3 | 50 |
The italics in AD-F1 and AD-R1 indicate BamHI and EcoRI restriction endonuclease sites, respectively.