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. 2014 Dec 3;9(12):e113585. doi: 10.1371/journal.pone.0113585

Table 1. Four oligonucleotides for the construction of the Aβ42 gene.

No. Sequence Length
AD-F1 5'-GGGGATCCGATGCAGAATTCCGACATGACTCAGGATATGAAGTTCATCA-3 49
AD-F2 5'-GATATGAAGTTCATCATCAAAAATTGGTGTTCTTTGCAGAAGATGTGGGT-3' 50
AD-R1 5'-GGCTCGAGCTACGCTATGACAACACCGCCCACCATGAGTCCAATGATTG-3 49
AD-R2 5'-ATGAGTCCAATGATTGCACCTTTGTTTGAACCCACATCTTCTGCAAAGAA-3 50

The italics in AD-F1 and AD-R1 indicate BamHI and EcoRI restriction endonuclease sites, respectively.