Skip to main content
. Author manuscript; available in PMC: 2015 Dec 5.
Published in final edited form as: Carbohydr Res. 2014 Sep 10;0:33–43. doi: 10.1016/j.carres.2014.08.016

Table 2.

IPRP-HPLC/UV conditions for isolation of tetrasaccharides from total tetrasaccharide fraction of enoxaparin

Column C18 Knauer 250 × 8 mm, 5 μm
Eluents Eluent A: 10 mM DBA, 10 mM CH3COOH in H2O-CH3OH = 43:57 (v/v)
Eluent B: 10 mM DBA, 10 mM CH3COOH in H2O-CH3OH = 50:50 (v/v)
Eluent C: 10 mM DBA, 10 mM CH3COOH in H2O-CH3OH = 80:20 (v/v)
Gradient  0 – 45 min isocratic step 100 % A
 45 – 45.2 min linear gradient from 100%A to 100%B
 45.2 – 60 min isocratic step 100 %B
 60 – 60.2 min linear gradient from 100%B to 100%C
 60.2 – 65 min isocratic step 100 %C
 65 – 65.2 min linear gradient from 100%C to 100%A
 65.2 – 90 min isocratic step 100 % A (column equilibrating)
Flow 2,5 ml/min
UV detection 232, 210 nm
Sample amount 1 mg