Skip to main content
. Author manuscript; available in PMC: 2015 Nov 10.
Published in final edited form as: Cancer Cell. 2014 Oct 30;26(5):695–706. doi: 10.1016/j.ccell.2014.09.009

Figure 1. Loss of Nf1 in embryonic DNSCs gives rise to classic plexiform neurofibroma in vivo.

Figure 1

(A) Diagram of experimental design: Isolation of DRGs/nerve roots from E13.5 Nf1flox/flox;R26R-LacZ embryos (a) and DNSCs culture (b); DNSCs were infected with Ad-Cre to ablate Nf1; Ad-GFP was used as a control (c); Nf1−/−;LacZ+ and Nf1flox/flox;LacZ− DNSCs were implanted to right and left sciatic nerves of nude mice, respectively (d).

(B) X-gal and H&E staining were perform on left (a and b) and right (c and d) sciatic nerve.

(C)Tumor on right sciatic nerve was stained with antibody against S100β and GAP43. Scale bars: 500 μm for blue scale bars, 50 μm for white and black scale bars.

See also Figure S1.