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. Author manuscript; available in PMC: 2014 Dec 3.
Published in final edited form as: Methods Cell Biol. 2014;121:141–153. doi: 10.1016/B978-0-12-800281-0.00010-5

Figure 10.3.

Figure 10.3

Soon after replacing the solution in the top “cell loading channel,” a gradient begins to form along the side channels. The droplet of fluid on top of the inlet (right) has to be cleaned before the tubing is removed.